1. Introduction/lab etiquettes/exam policy/visualizing yeast cells by light microscopy
2. Enhancing contrast in samples by staining:
(a) Gram staining/spore staining (eukaryotes)
3. Enhancing contrast in samples by staining: (b) Cells of peripheral blood by leishman staining. (Students will be pricking their fingers using sterile needles in aseptic condition to prepare blood smear.)
4. Introduction to cell fractionation: Isolation of chloroplasts from leaves
5. Introduction to gene organization: Understanding Lac Operon by Blue White screening.
6. Analysis and wrap-up Discussion on Practicals 1-5
7. Introduction to C.elegans Genetics - Stages of Development; students get hands on experience with manupulating C.elegans
8. Manipulating C.elegans - identify stages , males and hermaphrites.
9. Mutants - Recessive , dominant , temperature sensitive. These mutants will be used to Set up Genetic Crosses - Complete the genetic Cross and Analaysis.
10. RNAi – Students will use C.elegans to learn RNAi by feeding methods; Proper controls – efficiency of RNAi; Qualitiative and quantitative analysis ; Discuss about Setting up of Genome wide RNAi screens
11. Analysis of data obtained from the RNAi expt above . Plotting and presentation of data
12. Genome editing techniques - Focus on Crispr-Cas9 in C.elegans - demonstration and discussion ;
Analysis and wrap up Discussion on Practicals 7-12
anup.padmanabhan@ashoka.edu.in