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Biology Colloquium | Sander Tans

November 23 @ 1:30 pm - 2:30 pm

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Event Details

Title: Chaperoning single proteins

Abstract: Chaperones are critical to a properly functioning proteome. But studying how conformations of protein chains are affected is difficult, owing to inherent dynamics and heterogeneity. For this purpose, my lab has developed single-molecule techniques based on optical tweezers, and more recently in combination with single-molecule fluorescence detection. We have previously investigated the chaperones SecB, trigger factor, and Hsp70, which revealed unexpected capabilities to bind and stabilize intermediate folds – and thus act throughout the folding pathways.

In this talk, I will focus mainly on new experiments on the chaperone ClpB, which is known to reverse protein aggregation. Using a novel single-molecule approach, we show directly that ClpB extrudes loops of protein chains through its central pore. It also reveals notable processivity, power, step-dynamics, and switching between translocation modes. Protein extraction from aggregates thus can be highly deterministic and energy-driven process. In a second part, I will talk about recent work on the assembly of proteins into larger complexes. Here we use a combination of in-vivo and in-vitro approaches, specifically selective ribosomal profiling and single-molecule techniques. We show that protein dimers ubiquitously assemble during translation. It indicates that assembly can be coordinated in time and space, in contrast to diffusion-based assembly models.

Guest Session_Sander


Meeting ID: 949 6186 1666                                                         Passcode: 1P0qkP2J




November 23
1:30 pm - 2:30 pm


Department of Biology