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Role of daily dynamics of RNA binding proteins inbiological timekeeping of Chlamydomonas reinhardtii

Graduate Student Seminar Series | Dinesh Jadhav | Nov 9th, 2022

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Title:  Role of daily dynamics of RNA binding proteins in the biological timekeeping of Chlamydomonas reinhardtii


The Circadian clocks are endogenous, self-sustaining oscillators that regulate several biological processes according to the time of the day-night cycle. This circadian clock-driven regulation provides an adaptive advantage for the survival of organisms on the Earth. Transcriptional and translational feedback loops (TTFL) are the conserved oscillators underlying the endogenous clock. TTFLs are transcriptional oscillators that generate temporal RNA abundance, which was considered the primary driver for the generation of rhythmic physiology. However, recent reports have shown that the circadian clock can even persist without transcription and the TTFL oscillators. Additionally, a large number of studies suggest that circadian clock-driven physiological rhythms are also regulated at the post-transcriptional levels. RNA binding proteins (RBPs) play a crucial role in the regulation of multiple processes at post-transcriptional and translational levels, yet no study has reported the role of daily dynamics of RNA-RBPs interactome in the biological timekeeping of an organism. Here, we applied an unbiased high throughput RNA interactome capture in conjunction with state-of-the-art quantitative proteomics in Chlamydomonas reinhardtii across the 24 hours circadian cycle to identify and quantify RBPs. We identified several novel RBPs for the first time. Many of the RBPs are metabolic enzymes revealing their moonlighting role as RNA binding. Interestingly, many RNA-RBP interactions are changing in a time-of-day-dependent manner. Altogether, we envisage that the daily dynamics of RBP-RNA interaction will provide novel insights into circadian clock regulation of physiology by post-transcriptional mechanisms.

About the Speaker:

Dinesh Jadhav has a master's degree in Biotechnology from SRTM University. He learned basic molecular biology and proteomics techniques during his summer internship at CCMB Hyderabad. Currently, he is studying the post-transcriptional and translational regulation of the circadian clock in single-cell phytoplankton using molecular biology and high-throughput proteomics techniques

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