In the pursuit of deeper biological insights, imaging technologies like electron microscopy (EM) and X-ray microscopy (XRM) play pivotal roles. This workshop introduces the powerful synergy of these techniques and their impact on life sciences. EM-based approaches, including focused ion beam (FIB-SEM), serial block-face SEM (SBF-SEM), and array tomography, push past traditional imaging constraints. These advanced volume EM methods reveal intricate cellular architectures in nanometer resolution within a larger 3D context, enabling scientists to explore ultrastructural details with unprecedented clarity. Notably, SBF-SEM integrates an ultramicrotome within the SEM chamber, creating 15–30 nm thick sections for capturing high-resolution, 3D data of cellular structures.

This session will showcase practical applications, emphasizing how these imaging technologies drive advancements in life sciences and  explore integrated software solutions for image processing to further streamline and enrich data analysis.